A comparative immunochemical study of antisera to a tissue homogenate and a mixture of its nonprotein fractions
| Autor: | Perelaznyi Aa, Yu. V. Zykov, A. N. Kulagin, A. A. Nikitenko, N. A. Nazarenko, N. I. Skachkov, Mazina Nm, Gostev Vs, Saakov Ak, A. E. Azletskaya |
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| Rok vydání: | 1964 |
| Předmět: |
chemistry.chemical_classification
Antiserum Chromatography biology medicine.medical_treatment chemical and pharmacologic phenomena General Medicine Polysaccharide Complement fixation test General Biochemistry Genetics and Molecular Biology Titer Biochemistry chemistry Antigen medicine biology.protein Antibody Cytotoxicity Adjuvant |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 57:472-474 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00782518 |
| Popis: | Homogenate and a number of fractions free from soluble protein (lipid, polysaccharide, DNP) were obtained from the resected tissue of a human stomach cancer. Donkeys and horses were immunized with the homogenate or a mixture of the fractions mentioned with Freund's adjuvant. Gamma-globulin preparations were prepared from the antisera; their serological activity was studied in the complement fixation reaction with the test antigens — lipid, polysaccharide, DNP, saline extract. Immunization with the antigen of a simplified composition (fractions without soluble protein) led to the formation of antibodies to these fractions in a higher titer and with a less marked cytotoxicity than immunization with a more complicated tissue antigenic mixture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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