Autor:	Potekhina Ts, Sinitskaia Ns, Kas'ianov Ad, Shataeva Lk
Rok vydání:	2001
Předmět:	Fungal protein Chromatography biology Molecular mass Size-exclusion chromatography Saccharomyces cerevisiae biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Yeast Nucleoprotein Electrophoresis Nucleic acid
Zdroj:	Applied Biochemistry and Microbiology. 37:23-28
ISSN:	0003-6838
DOI:	10.1023/a:1002832122726
Popis:	Complexes of nucleic acids and acid nuclear proteins that are active toward human T-lymphocytes were isolated from cells of bakers' yeast Saccharomyces cerevisiae. The conditions of isolation of nucleoprotein complexes by acid extraction followed by microfiltration for concentration of macromolecular components were optimized. Gel filtration and electrophoresis were used to study the composition and molecular weights of components of the preparations obtained. It was shown that nucleoprotein complex had a molecular weight of 1430 kDa. However, only one zone was determined by electrophoresis of the protein component with a molecular weight of 30 kDa.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::24d8f564503e39666b6fa62661b8cc53 https://doi.org/10.1023/a:1002832122726 Zobrazit plný text záznamu Full text from SpringerLink