The role of subcellular localization in assessing the cytotoxicity of iodine-125 labeled iododeoxyuridine, iodotamoxifen, and iodoantipyrine
| Autor: | W. H. McLaughlin, D.E. Seitz, Ralph R. Weichselbaum, Stephen Adelstein, William D. Bloomer, Robert N. Hanson |
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| Rok vydání: | 1981 |
| Předmět: |
Chemistry
Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Antiestrogen Subcellular localization Pollution Molecular biology Analytical Chemistry chemistry.chemical_compound Nuclear Energy and Engineering Biochemistry Cytoplasm Cancer cell Nucleic acid Radiology Nuclear Medicine and imaging Thymidine Cytotoxicity Spectroscopy DNA |
| Zdroj: | Journal of Radioanalytical Chemistry. 65:209-221 |
| ISSN: | 1588-2780 0134-0719 |
| DOI: | 10.1007/bf02516105 |
| Popis: | There is abundant evidence that Auger effects from125I are singularly damaging if localized within DNA as the thymidine analogue125I-iododeoxyuridine (125IUdR). Recent work with125I-labeled intercalating agents and steroid sex hormones extends these observations by showing cytotoxicity with125I in sites other than the DNA backbone. We have compared the cytotoxicity of125IUdR,125I-iodotamoxifen, a non-steroidal antiestrogen that is translocated from the cytoplasm to the nucleus of receptor containing cells, and125I-iodoantipyrine, a biological indicator of the body water space, in human breast cancer cells (MCF-7) and report that cytotoxicity is critically dependent upon subcellular localization. When clonogenic survival of MCF-7 cells is expressed as a function of the concentration of125IUdR,125ITAM and125IAP in the culture media, the D37 values are 8·10−4, 2.3 and 68 μCi/ml, respectively. However, when survival is expressed as a function of the nucleic acid and protein subcellular fraction,125ITAM is just about as toxic as125IUdR localized within the DNA backbone. |
| Databáze: | OpenAIRE |
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