Tissue Extract Fluid Cytokine Levels as Markers for Wound Vitality
| Autor: | Adel F. Al-Kholy, Ahmed H. Rizk, Manal Hassan, Mohamed A. Mansour |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
business.industry medicine.medical_treatment 010401 analytical chemistry Interleukin 01 natural sciences Gastroenterology 0104 chemical sciences 03 medical and health sciences 0302 clinical medicine Cytokine Severe trauma Internal medicine embryonic structures Tissue extracts medicine 030216 legal & forensic medicine Surgical emergency business |
| Zdroj: | International Journal of Sciences. 2:87-94 |
| ISSN: | 2305-3925 2410-4477 |
| DOI: | 10.18483/ijsci.1055 |
| Popis: | Objectives: To evaluate ability of estimation of tissue extract fluid (TEF) levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6 for differentiation between antemortem (AM) and postmortem (PM) wounds and for rough determination of wound age. Materials & Methods: The study included 20 patients arrived to Surgical Emergency Department (ED) with severe trauma requiring surgical intervention and died after a known survival interval. Time elapsed since trauma inflection till arrival to ED was determined. Four skin biopsies were obtained from each patient: one AM and 3 PM specimens. Skin samples were homogenized and TEF was used for ELISA estimation of IL-1β, TNF-α and IL-6 levels. Results: AM TEF levels of IL-1β and TNF-α were significantly higher compared to PM levels with significantly higher levels in PM1 and PM3 specimens than PM2 specimens. TEF levels of IL-6 were significantly higher in PM2 compared to AM, PM1 and PM3 specimens with significantly higher levels in AM specimens compared to PM3 specimens. TEF levels of IL-1β and TNF-α showed positive significant correlation with wound vitality. TEF levels of IL-6 showed a positive significant correlation with time lapsed since wound inflection. Only elevated TEF level of IL-1β showed significantly high sensitivity for identification of wound inflected since ≤60 minutes AM Conclusion: TEF levels of IL-1β, TNF-α and IL-6 could differentiate between wounds inflected while victim was alive and PM wounds. TEF level of IL-1β works better for such differentiation and could determine wound inflected within 60 minutes with high sensitivity. |
| Databáze: | OpenAIRE |
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