Characterization of Tulbaghia violacea (Tulbaghieae, Allioideae, Amaryllidaceae) from India: a cytogenetic and molecular approach
| Autor: | Maumita Bandyopadhyay, Sreetama Bhadra, Abhishek Sadhu |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Nuclear gene biology Cell Biology Amaryllidaceae biology.organism_classification 010603 evolutionary biology 01 natural sciences Nuclear DNA Wild garlic Genus Botany Genetics Molecular Medicine Allioideae Internal transcribed spacer Molecular Biology Tulbaghia violacea 010606 plant biology & botany |
| Zdroj: | The Nucleus. 61:29-34 |
| ISSN: | 0976-7975 0029-568X |
| DOI: | 10.1007/s13237-017-0202-y |
| Popis: | Tulbaghia violacea, called ‘wild garlic’, is a native of Africa, though it is widely cultivated all over the world for its medicinal and horticultural importance. Absence of the genus from Indian floras and taxonomic surveys, as well as lack of proper molecular and cytogenetic characterizations, have instigated questions regarding origin and divergence of the Indian counterpart from its African relatives. In the present study, morphotaxonomical identification of Indian T. violacea was carried out, along with its karyomorphological characterization, 2C nuclear genome content determination, and analysis of ITS sequence. Mitotic study revealed that this species possessed 2n = 2x = 12 somatic chromosomes, two of which had secondary constrictions, whereas unstable meiotic chromosomal behavior was observed in flower buds, as reported earlier. 2C nuclear DNA content of T. violacea (44.51 ± 0.06 pg) was reported for the first time using flow cytometry, as was the ITS sequence data, from India. |
| Databáze: | OpenAIRE |
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