Optical improvements of a Z-shaped cell for high-sensitivity UV absorbance detection in capillary electrophoresis

Autor:	Remco E. J. van Soest, Stephen E. Moring, Richard T. Reel
Rok vydání:	1993
Předmět:	Absorbance Electrophoresis Capillary electrophoresis Optical path Optics Chemistry Capillary action business.industry Detector Sapphire business Order of magnitude Analytical Chemistry
Zdroj:	Analytical Chemistry. 65:3454-3459
ISSN:	1520-6882 0003-2700
Popis:	Absorbance detection in capillary electrophoresis (CE) offers excellent mass sensitivity but relatively poor concentration sensitivity. This dichotomy arises from the short optical path lengths found in current detector designs. Minimum detectable concentrations range from 10 -5 to 10 -7 M. The theoretical and physical design aspects affecting signal, noise, and optical transmission of various commercial (CE) detector cells are discussed. A new Z-shaped cell is described which uses a quartz ball lens to optimize light throughput in a 3-mm capillary section. The optical enhancement of the cell provides more than 1 order of magnitude improvement in the signal-to-noise ratio (MDC to 10 -8 M) over that of a conventional cell design with sapphire ball optics
Databáze:	OpenAIRE
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