Synthesis of fatty esters by polyethylene glycol-modified lipase
| Autor: | K. Ampon, Wan Md Zin Wan Yunus, Mahiran Basri, Che Nyonya Abdul Razak, A. Bakar Salleh |
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| Rok vydání: | 1995 |
| Předmět: |
Chromatography
biology Renewable Energy Sustainability and the Environment General Chemical Engineering Organic Chemistry Triacylglycerol lipase Chemical modification Chloroformate Polyethylene glycol Pollution Candida rugosa Inorganic Chemistry Solvent chemistry.chemical_compound Fuel Technology chemistry PEG ratio biology.protein Organic chemistry Lipase Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology AND Biotechnology. 64:10-16 |
| ISSN: | 1097-4660 0268-2575 |
| Popis: | Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activated PEG to the enzyme increased the degree of lipase modification. These modified lipases exhibited specific ester synthesis activities on organic solvents compared with native lipase. The degree of activity enhancement depended on the size of activated PEG used and the degree of modification of the enzyme. Maximal activity was attained after exhaustive of modification. The effects of different solvents, reaction temperature, and fatty acids on the esterification activity and the stability of the modified enzyme were investigated. The optimum esterification temperature (40° C) and preference of fatty acids as acyl donors of the modified lipase were very similar to those of the native enzyme. The modified lipase exhibited higher activity non-polar solvents than in polar solvents, and showed higher temperature, solvent and storage stability then the native lipase. |
| Databáze: | OpenAIRE |
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