A simple silver-staining technique for detecting Bence Jones proteins in unconcentrated urine

Autor:	J M Sheat, M A Lorier
Rok vydání:	1987
Předmět:	Immunofixation Gel electrophoresis Chromatography biology Chemistry Biochemistry (medical) Clinical Biochemistry Urine Biological fluid Bence Jones protein Staining Silver stain Biochemistry biology.protein Polyacrylamide gel electrophoresis
Zdroj:	Clinical Chemistry. 33:561-563
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/33.4.561
Popis:	Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.
Databáze:	OpenAIRE
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