Significance of acidification for measuring t-PA activity in human blood

Autor:	Masahiro Ieko, Yasukouchi T, Shoichi Nakagawa, Norihiro Satoh, Hiroshi Handa, Masahiro Satoh, Teiji Fujie, Yewki Thoma, Sakurama S
Rok vydání:	1988
Předmět:	Venous puncture Human blood Venous occlusion Activator (genetics) Immunology Specific activity Biological activity General Medicine Biology Pharmacology
Zdroj:	Blood & Vessel. 19:665-667
ISSN:	1884-2372 0386-9717
DOI:	10.2491/jjsth1970.19.665
Popis:	Our studies focused on the necessity of the immediate acidification for measuring the biological activity of tissue-plasminogen activator (t-PA) in the blood samples. The following results were obtained: 1. Biological Activity (BA) and immunological activity (IA) of t-PA increased after venous occlusion. However, the BA of t-PA was considerly lower in plasma than in the blood samples immediately acidified after venous puncture. 2. BA of t-PA in the blood decreased rapidly after sampling without acidification. 3. PAI activity in plasma correlated inversely to the specific activity of t-PA released venous occlustion.In conclusion, when measuring the t-PA activity in the blood, immediate acidification is necessary to prevent its inactivation probably by PAT co-existing in the plasma.
Databáze:	OpenAIRE
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