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Gut microbiota are recognized as an integral part in maintaining health, with disturbances in composition linked to disease states ranging from cancer to depression. Breast cancer patient microbiome analysis has demonstrated a correlation between dysbiosis and disease with proposed mechanisms including immune cell regulation, enhanced inflammatory signaling, and modulation of estrogen metabolism through the estrobolome. Current models lack the ability to study communication in one direction or to modulate single variables such as bacterial species or xenobiotic additions without the use of mouse models which suffer from poor translation and assessment opportunities. We have developed two microfluidic chips, one to model the gut microbiome using oxygen impermeable plastic for maintenance of anaerobic bacteria and another to culture breast cancer spheroids under constant flow. Current work has demonstrated viability and polarity maintenance in chip-cultured spheroids and initial development of polarized villus-like gut epithelium. Using these two chips, we will study the impact of breast cancer associated bacteria capable of estrogen metabolism on breast cancer tumorigenic potential. Citation Format: Danielle S. Brasino, Sean D. Speese, Beverly M. Emerson. Leveraging linked gut microbiome and breast cancer microfluidic chips to study the role of the estrobolome in cancer progression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 196. |
