Transfection of Mammalian Cells Using Block Copolypeptide Vesicles
| Autor: | Howard Dai, Daniel T. Kamei, Victor Z. Sun, Uh-Joo Choe, April R. Rodriguez, Timothy J. Deming |
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| Rok vydání: | 2013 |
| Předmět: |
animal structures
Polymers and Plastics viruses Immunogenicity Vesicle fungi Bioengineering Transfection Molecular biology Biomaterials Cell membrane chemistry.chemical_compound medicine.anatomical_structure chemistry Lipofectamine embryonic structures Materials Chemistry Biophysics medicine Magnet-assisted transfection Cytotoxicity DNA Biotechnology |
| Zdroj: | Macromolecular Bioscience. 13:539-550 |
| ISSN: | 1616-5187 |
| Popis: | An arginine-leucine block copolypeptide (R60 L20 ) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60 L20 vesicles also possess the ability to deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, the R60 L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. This behavior is potentially due to its stronger interaction with DNA which subsequently provides better protection against anionic heparin. |
| Databáze: | OpenAIRE |
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