Partial specific volume and molecular weight determinations from sedimentation equilibrium experiments in H2O and H218O

Autor:	S. Filitti-Wurmser, M. Tempête-Gaillourdet
Rok vydání:	1974
Předmět:	Aqueous solution Chromatography biology Diffusion Analytical chemistry General Medicine Lambda Biochemistry chemistry.chemical_compound chemistry Partial specific volume Immunoglobulin M Sedimentation equilibrium biology.protein Ultracentrifuge Guanidine
Zdroj:	Biochimie. 56:1183-1189
ISSN:	0300-9084
DOI:	10.1016/s0300-9084(74)80009-x
Popis:	Summary The partial specific volume ( ¯v 2 ) of a human (IgM) immunomacroglobulin type lambda has been measured by sedimentation equilibrium using the density perturbation method. With H 2 O and H 2 18 O as solvents, ¯v 2 was found to be 0.724 cm 3 /g. A value of 1 035 000 daltons was found for the molecular weight. The usual method of sedimentation-diffusion equilibrium has been applied to the study of this IgM in aqueous 5 M guanidine HCl. There is a weak preferential interaction for the guanidine, the apparent partial specific volume Φ' being 0.718 cm 3 /g.
Databáze:	OpenAIRE
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