| Autor: |
Pan, Yuan-Ming, Wang, Cheng-Gang, Zhu, Min, Xing, Rui, Cui, Jian-Tao, Li, Wen-Mei, Yu, De-Dong, Wang, Shu-Bin, Zhu, Wei, Ying-Jiang Ye, Wu, Yun, Wang, Shan, You-Yong Lu |
| Rok vydání: |
2016 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.c.3697450_d1.v1 |
| Popis: |
Oligonucleotide primers for STAT3, EZH2, β-actin, reporter gene, EMSA, and siRNA of STAT3. Table S2. Correlation between STAT3 and EZH2 expression in GC. Table S3. STAT3 and EZH2 were associated with the clinical stage of the malignancy. Table S4. Multivariate analysis of factors associated with OS. Figure S1. The EZH2 promoter-Luc constructs designed for this study. (A) schematic map of the short and full-length EZH2 promoter-Luc constructs, the full length of construct contained three important STAT3 binding motifs (-1702/+52), the short construct (-1702/-447) didn’t contain this STAT3 binding region (-436/+52). (B) The promoter sequence of EZH2 was located from -436 to +52. The three STAT3-binding motifs are highlighted in blue. Figure S2. Deletion analysis of EZH2 promoter activity. (A) Schematic maps of the EZH2 promoter 5′-deletion constructs used as reporter plasmids. (B) Relative luciferase activity after transient transfection of the reporter plasmids into SGC7901 cells. The ratios between the luciferase activities induced by each reporter vector and pRL-TK were calculated. The data shown are means ± SE of triplicate experiments. Figure S3. Kaplan-Meier analysis showed the combination between p-STAT3 and EZH2 expression in overall survival of GC patients. Figure S4. Relative expression of STAT3 by knocking down with specific siRNA in SGC7901 by real-time PCR analysis (**p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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