Protein Sensing Beyond the Debye Length Using Graphene Field-Effect Transistors
| Autor: | Malkolm Hinnemo, Patrik Ahlberg, Zhibin Zhang, Shi-Li Zhang, Jörgen Olsson, Asta Makaraviciute, Zhen Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Graphene Transistor Ionic bonding 02 engineering and technology Electrolyte 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences law.invention Dielectric spectroscopy symbols.namesake law Ionic strength Chemical physics symbols Electrical and Electronic Engineering 0210 nano-technology Instrumentation Debye length Debye |
| Zdroj: | IEEE Sensors Journal. 18:6497-6503 |
| ISSN: | 2379-9153 1530-437X |
| DOI: | 10.1109/jsen.2018.2849006 |
| Popis: | Sensing biomolecules in electrolytes of high ionic strength has been a difficult challenge for field-effect transistor-based sensors. Here, we present a graphene-based transistor sensor that is capable of detection of antibodies against protein p53 in electrolytes of physiological ionic strength without dilution. As these molecules are much larger than the Debye screening length at physiological ionic strengths, this paper proves the concept of detection beyond the Debye length. The measured signal associated with the expected specific binding of the antibodies to p53 is concluded to result from resistance changes at the graphene-electrolyte interface, since a sensor responding to resistance changes rather than charge variations is not limited by Debye screening. The conclusion with changes in interface resistance as the underlying phenomena that lead to the observed signal is validated by impedance spectroscopy, which indeed shows an increase of the total impedance in proportion to the amounts of bound antibodies. This finding opens up a new route for electrical detection of large-size and even neutral biomolecules for biomedical detection applications with miniaturized sensors. |
| Databáze: | OpenAIRE |
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