Experimental and theoretical studies of the interactions of some synthetic food dyes with human serum albumin

Autor:	Seyedeh Maedeh Majidi, Mohammad Reza Hadjmohammadi, Parvin Hoseyni, Mohammad Hossein Fatemi
Rok vydání:	2021
Předmět:	Chromatography Hsa binding Ether General Chemistry Intrinsic fluorescence Human serum albumin Binding constant Fluorescence spectroscopy body regions Residue (chemistry) chemistry.chemical_compound chemistry In vitro system embryonic structures medicine medicine.drug
Zdroj:	Journal of the Iranian Chemical Society. 19:885-892
ISSN:	1735-2428 1735-207X
DOI:	10.1007/s13738-021-02351-4
Popis:	The interaction of 10 synthetic food dyes with human serum albumin (HSA) was investigated using fluorescence spectroscopy, biopartitioning micellar chromatography (BMC) and molecular docking studies. The results obtained by fluorescence spectroscopy indicated that these dyes could strongly quench the intrinsic fluorescence of HSA due to interaction with Trp-214 residue of HSA. The binding constant (Kb) and the number of HSA binding sites were calculated using modified Stern–Volmer equation. BMC as a micellar mobile phase is used as an in vitro system by polyoxyethylene 23 lauryl ether (Brij-35) to predict the Kb of food dyes to HSA. The relationship between the BMC retention data and Kb of food dyes was modeled and the predictive ability of model evaluated. The correlation coefficient (R) and standard error (SE) of developed model were 0.928 and 1.994, respectively. Moreover, molecular docking explorations show that interaction occurs in the subdomain IIA (site I) of HSA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::24221caaf11c2e9c41b369ad218088e5 https://doi.org/10.1007/s13738-021-02351-4 Zobrazit plný text záznamu Full text from SpringerLink