Separation and quantification of components of edible fat utilizing open tubular columns in SFC. Sample introduction by direct injection and SFE coupled on-line to SFC
| Autor: | Bjørn E. Berg, H. S. Lund, Tyge Greibrokk |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Detection limit
animal structures Chromatography Organic Chemistry Clinical Biochemistry Trimyristin Extraction (chemistry) Analytical chemistry Supercritical fluid extraction Biochemistry Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound chemistry Tripalmitin Supercritical fluid chromatography Unsaturated fatty acid |
| Zdroj: | Chromatographia. 44:399-404 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02466317 |
| Popis: | Geometric isomers of fatty acids were separated by open tubular columns in SFC. The concentration of the analytes varied between 9 and 16 μg mL−1. Quantification of triglycerides with repeatability better than 20% were obtained in a home made SFE-SFC unit. A four point calibration curve for both trimyristin and tripalmitin was developed with correlation coefficients of 0.998 and 0.9998, respectively. The limit of quantification was approximately 3 ng for both components. Supercritical CO2 as extraction solvent in matrix containing lipids increased the recovery of cholesterol by a factor of three. Using immobilized lipase in on-line SFE-SFC quantification of cholesterol in spray dried egg yolk was possible. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink