Tetranucleotide microsatellites from the loggerhead sea turtle (Caretta caretta)
Autor: | John P. Carroll, C. Joseph Nairn, Steven B. Castleberry, Brant C. Faircloth, Alicia Wood-Jones, Brian M. Shamblin, Mark G. Dodd |
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Rok vydání: | 2007 |
Předmět: |
Ecology
biology Zoology Locus (genetics) biology.organism_classification Biochemistry Loggerhead sea turtle General Biochemistry Genetics and Molecular Biology law.invention body regions Loss of heterozygosity Sea turtle law Microsatellite human activities Polymerase chain reaction Tetranucleotide Repeats |
Zdroj: | Molecular Ecology Notes. 7:784-787 |
ISSN: | 1471-8286 1471-8278 |
DOI: | 10.1111/j.1471-8286.2007.01701.x |
Popis: | We describe primers and polymerase chain reaction conditions to amplify 15 tetranucleotide microsatellite loci from the loggerhead sea turtle ( Caretta caretta ). The primers were tested on 30 individuals that nested along the Georgia, USA coast. The primer pairs developed in this study yielded an average of 13.9 alleles per locus (range of 10–21), an average observed heterozygosity of 0.91 (range 0.79–1.00), and an average polymorphic information content of 0.88 (range 0.84–0.92). |
Databáze: | OpenAIRE |
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