Autoclaved Polyethylene Glycol decreases yeast protoplast reversion and hybrid production

Autor:	Peter A. Whittaker, Mahmoud A Ghannoum, Kevin Kavanagh, I. Mansour
Rok vydání:	1990
Předmět:	Chromatography fungi technology industry and agriculture Acetaldehyde Reversion macromolecular substances Polyethylene glycol Protoplast Polyethylene Applied Microbiology and Biotechnology Biochemistry Yeast chemistry.chemical_compound Membrane chemistry PEG ratio
Zdroj:	Biotechnology Techniques. 4:281-284
ISSN:	1573-6784 0951-208X
DOI:	10.1007/bf00158844
Popis:	Autoclaved solutions of the fusogen Polyethylene Glycol (PEG) give reduced yeast protoplast reversion and hybrid formation. Autoclaving has been shown to cause PEG degradation and increase the acetaldehyde content. Consequently, membrane filtration is recommended as the optimum means of sterilising PEG solutions to ensure maximum protoplast reversion and hybrid yield.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::23f46e981d34230f38d8cea72720db28 https://doi.org/10.1007/bf00158844 Zobrazit plný text záznamu Full text from SpringerLink