| Autor: |
Kamal Sarma, P. Suresh Babu, Abhay Kumar, M. Makesh, Deepika Anand, K. V. Rajendran, Saurav Kumar, Raju Baitha, Snatashree Mohanty |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Indian Journal of Fisheries. 67 |
| ISSN: |
0970-6011 |
| DOI: |
10.21077/ijf.2019.67.2.84594-08 |
| Popis: |
Serum immunoglobulins (Ig) of mrigal Cirrhinus mrigala (Hamilton 1822) immunised with bovine serum albumin (BSA), were purified by affinity chromatography using BSA-CL agarose column. The purified mrigal Ig (m-Ig) was characterised under reducing condition by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) which revealed two bands of 85 and 26 kDa corresponding to heavy and light chain, respectively. Following fusion of splenocytes from Balb/c mice immunised with purified m-Ig with myeloma cells, three hybridomas showing reactivity with m-Ig were cloned by limiting dilution. The monoclonal antibodies (MAbs) generated by these clones were designated as 3B2-E12, 3B2-F9 and 4C3-B2 and characterised by western blotting and isotyping. Western blot analysis of the supernatant from the three clones with purified m-Ig indicated that, all the three MAbs were specific to heavy chain. Isotyping revealed that 3B2-E12 MAb was of IgG1 isotype whereas the other two MAbs were of IgG2a isotype. Cross reactivity of anti-mrigal Ig MAb (3B2-E12) was observed with serum Ig of Catla catla and Labeo rohita indicating semi-conserved nature of Ig in Indian major carps. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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