Cryopreservation of Azotobacteraceae in liquid nitrogen
| Autor: | J. P. Thompson |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
biology
Physiology Azotobacter beijerinckii Plant Science General Medicine Liquid nitrogen biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Microbiology Cryopreservation Ampoule Horticulture chemistry.chemical_compound chemistry Botany Glycerol Desiccation Bacteria Food Science Biotechnology Azotobacteraceae |
| Zdroj: | MIRCEN Journal of Applied Microbiology and Biotechnology. 3:323-336 |
| ISSN: | 1573-0972 0265-0762 |
| DOI: | 10.1007/bf00933586 |
| Popis: | Agar-grown cultures of 144 strains of Azotobacteraceae were suspended in 0.5 ml of 10% (v/v) glycerol in glass ampoules and rapidly frozen and stored in liquid nitrogen. On revival, ampoules were rapidly warmed by agitation in a waterbath at 33°C until thawing occurred. All cultures remained viable for up to 7.5 years, grew with short lag phases on revival and suffered no apparent phenotypic changes. Quantitative assessments of representative strains of all species suffered losses in viability after 2.5 or 5 years in liquid nitrogen that were generally less than one decimal reduction from unfrozen suspensions of 107–109 cells/ml. The method is considerably better than freeze-drying or simple desiccation for the more-difficult species likeAzomonas insignis, A. agilis, Azotobacter beijerinckii, Azomonotrichon macrocytogenes andDerxia gummosa, and indications are that strains of all species could be preserved in this way for decades. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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