Comparative Study of Bunt Pathogen Resistance to the Effects of Fungicides in Callus Co-Cultures Triticum aestivum with Tilletia caries

Autor:	O. B. Surina, L. M. Yarullina, R. I. Ibragimov, B. R. Kuluev, R. I. Kasimova, Liubov G. Yarullina
Rok vydání:	2014
Předmět:	Fungicide Horticulture Tissue culture biology Callus General Medicine Tilletia caries Pesticide biology.organism_classification Gene Pathogen 18S ribosomal RNA Microbiology
Zdroj:	Agricultural Sciences. :906-912
ISSN:	2156-8561 2156-8553
DOI:	10.4236/as.2014.510098
Popis:	The morphophysiological and molecular-genetic parameters of T. caries isolates collected from various fields of Southern Urals agrocenoses have been analysed. Isolate 1 of wheat callus had a high growth rate in vitro even in the presence of 0.1 mg/l fungicide Baitan. Isolate 2 of wheat callus had a low growth rate whereas 0.1 mg/l Baitan significantly inhibited its growth. Comparison of nucleotide sequences of 18S RNA gene of the two isolates showed high level of homology between them, but a large number of nucleotide substitutions have been found. The most characteristic excision was five nucleotides at position 461 of the isolate 2, compared with the isolate 1. Our results allow to assume that the environmental stress including high pesticide concentration may cause the resistance of T. caries pathogen to fungicides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::23d9e9dd41b25d5bcde5f5f071e3d7ba https://doi.org/10.4236/as.2014.510098 Zobrazit plný text záznamu