Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes

Autor:	Alfredo Giménez-Cassina, Nika N. Danial, Sofia M. Ribeiro
Rok vydání:	2014
Předmět:	Cellular bioenergetics Chemistry Cellular respiration Extramural Extracellular chemistry.chemical_element Cortical neurons Oxygen Cell biology
Zdroj:	Methods in Molecular Biology ISBN: 9781493918744
DOI:	10.1007/978-1-4939-1875-1_6
Popis:	The introduction of microplate-based assays that measure extracellular fluxes in intact, living cells has revolutionized the field of cellular bioenergetics. Here, we describe a method for real time assessment of mitochondrial oxygen consumption rates in primary mouse cortical neurons and astrocytes. This method requires the Extracellular Flux Analyzer Instrument (XF24, Seahorse Biosciences), which uses fluorescent oxygen sensors in a microplate assay format.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::23c99bb6e626e4e2e77722387e111adf https://doi.org/10.1007/978-1-4939-1875-1_6 Zobrazit plný text záznamu