Comparative study of lactic acid and polylactides using static headspace, gas chromatography and high-performance liquid chromatography
| Autor: | M. O'Hanlon, T.M. Khong, M. Staniforth |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Organic Chemistry General Medicine Mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry Lactic acid law.invention chemistry.chemical_compound chemistry law Sodium lactate Flame ionization detector Sample preparation Gas chromatography Electron ionization |
| Zdroj: | Journal of Chromatography A. 833:195-208 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(98)00911-x |
| Popis: | This work investigates the use of analytical methodology to determine lactic acids and/or polylactides. This paper reports three methods that have been successfully developed. Firstly, static headspace gas chromatography (GC) was investigated. The results show precision values of 4–5% RSD and linearity correlation coefficients of between 0.990 and 0.999 in the concentration range 300–700 μg/ml lactic acid. The method is specific for lactic acid. Secondly, an on-column GC method is presented which minimises sample preparation and separation of lactic acid from the polylactides. Finally, an ion-exclusion high-performance liquid chromatography (HPLC) method was developed. The instrumental precision was 0.1%, and linearity correlation coefficients >0.9999 were obtained in the concentration range between 50 and 200 μg/ml lactic acid. A basic hydrolysis is used to convert the polylactides to lactic acid. Collectively, these methods form a successful approach for the determination of lactic acid in consumer healthcare products. |
| Databáze: | OpenAIRE |
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