Human umbilical cord blood γδ T cells expanded ex vivo with zoledronic acid are distinct from human peripheral blood γδ T cells (HEM2P.244)
| Autor: | Suzanne Tomchuck, Scarlett Evans, Ross Perko, Jin He, Amy McKenna, Pradyot Dash, Paul Thomas, Mari Dallas |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 194:51.14-51.14 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.194.supp.51.14 |
| Popis: | Ex vivo expansion of human peripheral blood (PB) γδ T cells using aminobisphosphonates has been extensively studied. However, as human umbilical cord blood (CB) γδ T cells are not as well described, we conducted a detailed comparison of proliferation, phenotype, TCR repertoire and function of CB and PB γδ T cells cultured with zoledronic acid. The percent of γδ T cells within the lymphocyte subset significantly increased in both samples after culture, but CB γδ T cells proliferated more than PB. Phenotypically, Vγ9Vδ2 was a common clone in fresh PB γδ T cells, which predominated after culture, yet the number of CB Vγ9Vδ2 cells remained low. The memory subsets of fresh γδ T cells were similar, but after culture, PB γδ T cells became traditional memory cells while CB γδ T cells were intermediate CD45RA+CD45RO+ memory cells. Memory status corresponded with TCRγδ variant; more Vγ9 and Vδ2 cells were memory while Vδ1 cells were naïve. Functionally, PB and CB γδ T cells had distinct cytokine secretion profiles. PB γδ T cells secreted more IFN-γ and TNF-α after culture, while CB γδ T cells secreted more IL-10. Using a single cell multiplex PCR assay, we found the TCRγδ repertoire of fresh cells to be diverse in both samples, yet CB γδ T cells maintained diversity after culture. Our results suggest that zoledronic acid may not stimulate γδ T cells in CB the same as PB and analysis of the TCRγδ variants may give insight into the difference in γδ T cell function of these samples. |
| Databáze: | OpenAIRE |
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