A polymyxin-cloth enzyme immunoassay system for detection ofEscherichia coliO157 in ground beef
| Autor: | B.W. Blais, R.A. Booth, R.P. Johnson, Hiroshi Yamazaki |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology medicine.diagnostic_test medicine.drug_class Polymyxin biology.organism_classification medicine.disease_cause Microbiology Enterobacteriaceae Antigens present Enzyme chemistry Polyclonal antibodies Immunoassay medicine biology.protein lipids (amino acids peptides and proteins) Escherichia coli Bacteria Food Science |
| Zdroj: | Food Microbiology. 13:237-242 |
| ISSN: | 0740-0020 |
| DOI: | 10.1006/fmic.1996.0029 |
| Popis: | A polymyxin-cloth enzyme immunoassay (polymyxin-CEIA) was developed for the detection of the foodborne pathogenEscherichia coliO157. This simple assay utilized inexpensive polymyxin-coated polyester cloth as an adsorbent forE. colilipopolysaccharide (LPS) antigens present in test samples such as enrichment cultures of ground beef. Captured LPS was immunoenzymatically detected using commercially available anti-O157 polyclonal antibodies. The assay was sensitive and highly specific for strains bearing the O157 and related antigens, and permitted the detection ofc. 1–10 cfus spiked into ground beef samples after a brief enrichment in double modified trypticase soy broth. We suggest that the polymyxin-CEIA will provide a simple, rapid and inexpensive screening method forE. coliO157 in ground beef. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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