Overexpression of the 77-kD echinoderm microtubule-associated protein (EMAP), a WD-40 repeat protein, in baculovirus-infected Sf9 cells

Autor:	Robert F. Weaver, Melissa A. F. Daggett, Kathy A. Suprenant, Qingwen Li
Rok vydání:	1998
Předmět:	biology Microtubule-associated protein viruses Sf9 Cell Biology biology.organism_classification Strongylocentrotus purpuratus Molecular biology Cell biology Spindle apparatus law.invention Tubulin Structural Biology Microtubule law biology.protein Recombinant DNA Cytoskeleton
Zdroj:	Cell Motility and the Cytoskeleton. 41:57-67
ISSN:	1097-0169 0886-1544
DOI:	10.1002/(sici)1097-0169(1998)41:1<57::aid-cm5>3.0.co;2-c
Popis:	The purpose of this study was to test whether any assembly-promoting microtubule-associated protein (MAP) would bundle microtubules and induce process formation in recombinant baculovirus-infected Sf9 cells, in particular, whether a non-neural MAP from a normally rounded cell would produce cellular asymmetries. To carry out these experiments, we constructed a recombinant baculovirus that expressed the full-length 77-kD EMAP, an abundant MAP that localizes to the mitotic spindle of cleavage-stage sea urchin embryos and to the interphase array of microtubules in adult coelomocytes. Expression of EMAP in Sf9 cells had no detectable effect on cellular morphology, microtubule organization, or stability. These results indicate that process formation in Sf9 cells is MAP specific.
Databáze:	OpenAIRE
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