64 EFFECTS OF NANOPURIFIED BOAR SEMEN FOR ARTIFICIAL INSEMINATION ON PROTEIN DETECTION IN SWINE OFFSPRING MUSCLE AND FAT TISSUE
| Autor: | Jean M. Feugang, D. Devost-Burnett, Peter L. Ryan, W. A. Moorhead, Shengfa F Liao, C. L. Durfey, Scott T. Willard, G. D. A. Gastal |
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| Rok vydání: | 2017 |
| Předmět: |
Muscle tissue
medicine.medical_specialty medicine.diagnostic_test Offspring Adipose tissue Semen Reproductive technology Biology Immunofluorescence Sperm Endocrinology medicine.anatomical_structure Reproductive Medicine Internal medicine Genetics medicine Animal Science and Zoology Molecular Biology Spermatogenesis Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 29:139 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv29n1ab64 |
| Popis: | Standard extended semen contains both viable and non-viable spermatozoa. Magnetic nanoparticles have proven to be effective in the purification of boar semen by targeting nonviable spermatozoal cells (Feugang et al. 2015 IVF Reprod. Med. Genet. 3, 2), allowing potentially greater efficiency within the pork production industry. Previous research lacks data regarding the biochemical effects on offspring produced from such nanopurified semen. Here, we aim to determine whether there is a difference in protein expression between offspring produced with standard and nanopurified semen. Myoglobin (MYO) and fatty acid synthase (FAS) were chosen as the protein markers for this study because they are often studied in reference to meat quality. Myoglobin provides meat its red colour, and FAS assembles fatty acids contributing to tenderness and palatability. The results produced here will provide a baseline for further research in the meat quality and the safety of consuming meat produced with this nanopurification method. Six sows maintained on our experimental farm were inseminated with standard (CTRL) and nanopurified (NANO) boar semen, leading to the birth of viable full-term piglets. At weaning, 10 pigs (5 male and 5 female) were randomly selected from each group and allowed to grow to market weight. Samples of longissimus muscle and SC back fat were then collected from each pig and prepared for protein analyses. Western immunoblotting and immunofluorescence of tissue samples were performed using anti-MYO and anti-FAS antibodies. Images were appropriately captured and quantified (ImageJ). Data (mean ± SEM) were analysed (ANOVA/Wilcoxon) with P |
| Databáze: | OpenAIRE |
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