Congenital transmission of Mexican strains of Trypanosoma cruzi TcIa: interaction between parasite and human placental explants

Autor: Luis Eduardo Ramirez, Tamires Marielem Carvalho-Costa, Paula Tatiana Mutão Ferreira, Marcos Vinicius da Silva, Ana Carolina Morais de Oliveira, Karine Rezende de Oliveira, Carlo José Freire Oliveira, César Gómez Hernández, Virmondes Rodrigues, Fernanda Rodrigues Helmo, Cecilia Gomes Barbosa, Lorena Kelly Alves Saraiva, Chamberttan Souza Desidério, Eloisa Amália Vieira Ferro, Ingrid Ketlen Pereira Dos Santos, Juliana Reis Machado
Rok vydání: 2021
Předmět:
Zdroj: Parasitology. 149:418-426
ISSN: 1469-8161
0031-1820
Popis: Congenital transmission of Chagas disease plays an important role in endemic countries because it is not a diagnosis that is encountered frequently in prenatal care. Due to limited information regarding congenital transmission of Trypanosoma cruzi in Mexico, the present study aimed to investigate protozoan infectivity and modulation of immune responses in human placental explants infected with T. cruzi Ia Mexican strains. The Inc-5 strain showed increased infectivity and modulated IL-1β, IL-10 and TLR-4, decreasing their expression after 24 h of infection. Both strains (Inc-5 and Ninoa) stimulated the production of TNF-α and decreased IL-6 levels 96 h after infection. An important detachment of the syncytiotrophoblast caused by infection with T. cruzi was observed after 24 h of infection. In this study, ex vivo infection of human placental villi was performed to better understand interactions involving parasitic T. cruzi and human placental tissue. It was concluded that the strains of TcIa present parasitism in placental tissue, modulation of the innate immune system of the placenta, and cause intense detachment of the syncytiotrophoblast, a fact that may be more associated with abortion and premature birth events than the congenital transmission itself, justifying the low rate of this transmission mechanism by this genotype.
Databáze: OpenAIRE
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