Cytokine Induction in Experimental and Ulcerative Colitis
| Autor: | S. A. Radema, Daan W. Hommes, G. N. J. Tytgat, Y. Zhao, Arnold Stronkhorst, Jeroen M. Jansen, S. J. H. Van Deventer, Paul Fockens |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty Lamina propria business.industry medicine.medical_treatment Gastroenterology In situ hybridization medicine.disease Ulcerative colitis Inflammatory bowel disease Cytokine medicine.anatomical_structure medicine Surgery Tumor necrosis factor alpha Colitis business Acute colitis |
| Zdroj: | Digestive Surgery. 9:177-182 |
| ISSN: | 1421-9883 0253-4886 |
| Popis: | In order to delineate the role of cytokines in inflammatory bowel disease, we studied induction of these mediators in an acetic acid-induced rat model for colitis. In this model, IL-1β mRNA induction was detected early after induction of acute colitis. Using in situ hybridization techniques, enterocytes were identified as the predominant source of IL-1β mRNA. TNFα mRNA was detected with in situ hybridization in cells located in the lamina propria and in the submucosa. Immunofluorescent techniques showed cells with the same localisation producing TNFα protein. Cultured human colon biopsies from patients with ulcerative colitis secreted large amounts of interleukin-1 but only modest quantities of tumor necrosis factor. Thus, interleukin-1 is synthesized in experimental as well as ulcerative colitis, and may be causally related to its pathogenesis. These findings open new possibilities for development of intervention strategies in ulcerative colitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|