Preparation of K+-Doped Core-Shell NaYF4:Yb, Er Upconversion Nanoparticles and its Application for Fluorescence Immunochromatographic Assay of Human Procalcitonin
| Autor: | Hongman Zhang, Yuwang Han, Jie Tang, Hui Feng, Lijiang Lei |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Ammonium bromide Chromatography Sociology and Political Science Hydrochloride Clinical Biochemistry Aqueous two-phase system 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Polyelectrolyte Photon upconversion 0104 chemical sciences Allylamine Clinical Psychology chemistry.chemical_compound chemistry 0210 nano-technology Law Spectroscopy Social Sciences (miscellaneous) Acrylic acid Nuclear chemistry |
| Zdroj: | Journal of Fluorescence. 26:2237-2246 |
| ISSN: | 1573-4994 1053-0509 |
| DOI: | 10.1007/s10895-016-1919-8 |
| Popis: | In the present study, we reported a convenient route to prepare well dispersed and functionalized K+-doped core-shell upconversion nanoparticles (UCP) by layer-by-layer (LbL) assembly of polyelectrolytes. UCP was firstly transferred to aqueous phase using cationic surfactant cetyl trimethyl ammonium bromide (CTAB) via hydrophobic interaction without removing the existing oleic acid (OA). Then the positively charged hydrophilic UCP@CTAB was further alternately deposited with negatively charged [poly (sodium 4-styrenesulfonate)] (PSS), positively charged [poly (allylamine hydrochloride)] (PAH) and negatively charged [poly (acrylic acid)] (PAA). The final carboxyl functionalized UCP@CTAB@PSS@PAH@PAA was then conjugated with monoclonal antibody1 (AB1) of procalcitonin (PCT), resulting in successful detection of PCT antigens based on the immunochromatographic assay (ICA). Linear response was achieved from 0 to 10 ng/mL, and the lowest limit of detection (LLD) was 0.18 ng/mL. |
| Databáze: | OpenAIRE |
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