Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry

Autor:	Colette F. Quinn, Lee D. Hansen
Rok vydání:	2019
Předmět:	0303 health sciences Chemistry 030302 biochemistry & molecular biology Enthalpy Kinetics Thermodynamics Isothermal titration calorimetry Calorimetry Michaelis–Menten kinetics Heat capacity rate Reaction rate 03 medical and health sciences Enzyme kinetics 030304 developmental biology
Zdroj:	Methods in Molecular Biology ISBN: 9781493991785
DOI:	10.1007/978-1-4939-9179-2_17
Popis:	This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::235a3cbe46aab4d790c708b29ed29635 https://doi.org/10.1007/978-1-4939-9179-2_17 Zobrazit plný text záznamu