Enhanced two-electrode photoelectrochemical biosensing platform amplified by bilirubin oxidase labelling
| Autor: | Shiting Gu, Xueyu Lu, Gao-Chao Fan, Yibin Lu, Xiao-Mei Shi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Aptamer Metals and Alloys Substrate (chemistry) Nanoparticle Nanotechnology 02 engineering and technology Carbon nanotube 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention law Electrode Materials Chemistry Electrical and Electronic Engineering 0210 nano-technology Bilirubin oxidase Selectivity Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 343:130060 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2021.130060 |
| Popis: | Herein, a novel and robust two-electrode photoelectrochemical (PEC) biosensing platform amplified by bilirubin oxidase (BOD) labelling was reported. This two-electrode operating system was composed of photoanode and sensing biocathode. Carcinoembryonic antigen (CEA) was utilized as a model for probing. Specifically, Fe3+-doped TiO2 (Fe:TiO2) nanotubes were first prepared and then deposited with ZnIn2S4 (ZIS) nanocrystals to develop the ZIS/Fe:TiO2 photoanode. Toward sensing biocathode, after carbon nanotubes (CNTs) and Au nanoparticles (NPs) were modified on conductive glass, an Au/CNT cathodic substrate was fabricated to anchor the CEA aptamer. Complementary DNA (cDNA) of the CEA aptamer was labelled with BOD to form the cDNA-BOD conjugate that amplified the signal through DNA hybridization. Target detection hinged on an evident decline in the current signal generated by terminated amplification of the BOD labels and evident steric hindrance of the captured CEA molecules. Profiting from excellent PEC property of the ZIS/Fe:TiO2 photoanode and good oxygen-reduction capability of the BOD labels, the two-electrode PEC biosensing platform exhibited high sensitivity. Because of the division of biorecognition with photoanode, the platform also showed satisfactory selectivity in biological matrix. This elegant two-electrode strategy offers a promising path for seeking other robust PEC biosensors applying in complex biological samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect