Lipase from Candida paralipolytica. VII. Detection of lipase-inhibiting substances in Candida paralipolytic and inhibition by phospholipids of Candida paralipolytica lipase
| Autor: | Yasuhide Ota, Yasuji Minoda, Tsutomu Sugiura, Koichi Yamada |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Phosphatidylethanolamine
Chromatography biology Sodium chemistry.chemical_element Calcium Glycerophospholipids General Biochemistry Genetics and Molecular Biology Yeast chemistry.chemical_compound chemistry Biochemistry Phosphatidylcholine biology.protein Phosphatidylinositol Lipase General Agricultural and Biological Sciences |
| Zdroj: | Agricultural and Biological Chemistry. 39:51-56 |
| ISSN: | 1881-1280 0002-1369 |
| Popis: | As a result of searching for a lipase-inhibiting substances in the cells and in the culture medium of Candida paralipolytica, it was shown that several glycerophospholipids from the cells inhibited the activity of the lipase from the yeast. Three phospholipids were identified as phosphatidylethanolamine (PE), phosphatidylcholine and phosphatidylinositol by thin-layer chromatography.At pH 8.2, all the phospholipids tested were inhibitory, whereas at pH 6.0, at which the yeast was usually cultured, only PE was inhibitory.Relationship between the inhibition by phospholipids and calcium ions, and effect of the concentration of sodium chloride on the inhibition with PE were also reported. |
| Databáze: | OpenAIRE |
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