Specific protein-membrane interactions promote the export of metallo-β-lactamases via outer membrane vesicles
| Autor: | Bahr G, Dal Peraro M, Alessio Prunotto, Lisandro J González, Carolina López, Alejandro J. Vila, Robert A. Bonomo |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
biology Vesicle biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification medicine.disease_cause Membrane Enzyme chemistry polycyclic compounds medicine Biophysics Secretion Bacterial outer membrane Beta (finance) Escherichia coli Bacteria |
| Popis: | Outer membrane vesicles (OMVs) act as carriers of resistance determinants such as metallo-β-lactamases. The metallo-β-lactamase NDM-1 is present in OMVs produced by Gram-negative bacteria since it is a lipidated, membrane-anchored protein. The soluble domain of NDM-1 also forms electrostatic interactions with the membrane. Herein, we show that these interactions promote its export into OMVs produced by Escherichia coli. We report that favorable electrostatic protein-membrane interactions are also at work in the soluble enzyme IMP-1, while being absent in VIM-2. These interactions correlate with an enhanced secretion into OMVs of IMP-1 compared to VIM-2. Disruption of these interactions in NDM-1 and IMP-1 impairs export into vesicles, confirming their role in defining the protein cargo in OMVs. These results also indicate that export of metallo-β-lactamases into vesicles in their active form is a common phenomenon that involves cargo selection based on molecular features. |
| Databáze: | OpenAIRE |
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