LATE-BREAKING ABSTRACT: Hypoxia-inducible 5-eicosatetraenoates are potential metabolite markers for screening obstructive sleep apnea
| Autor: | Hyun Woo Shin, Jong Wan Park, K.H. Cho, Chae-Seo Rhee, Il-Hee Hong, Joo Youn Cho, Daeho So |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Creatinine medicine.diagnostic_test business.industry medicine.medical_treatment Metabolite Intermittent hypoxia Urine Polysomnography Hypoxia (medical) medicine.disease Gastroenterology respiratory tract diseases Obstructive sleep apnea chemistry.chemical_compound Endocrinology chemistry Internal medicine medicine Continuous positive airway pressure medicine.symptom business |
| Zdroj: | 4.2 Sleep and Control of Breathing. |
| DOI: | 10.1183/13993003.congress-2016.pa2302 |
| Popis: | Background: Early detection of obstructive sleep apnea (OSA) is needed to reduce cardiovascular sequelae and mortality. Aims: To develop an alternative diagnostic method for OSA, we aimed to search for OSA markers reflecting hypoxic stress during sleep. Methods: We collected the first morning urine from 63 healthy male controls and from 80 male OSA patients who had been newly diagnosed in a tertiary referral center and a private specialized clinic. We performed metabolome-wide analysis to find and validate surrogate markers for OSA diagnosis in different cohorts, and examined the correlation between urinary levels of the metabolic markers and laboratory findings on polysomnography. The molecular pathway of these metabolites was investigated in vitro and in vivo. Results: Arachidonic acid derivatives 5-HETE and 5-oxoETE were detected in urine samples. The levels (mean±SD, ng per mg creatinine) of 5-HETE and 5-oxoETE were 56.4±26.2 and 46.9±18.4 in OSA patients, respectively, which were significantly higher than those in controls (22.5±4.6 and 18.7±3.6). Both levels correlated with the apnea-hypopnea index and the lowest oxygen saturation on polysomnography. After OSA patients were treated with the continuous positive airway pressure, the metabolite levels were significantly reduced. In cultured mononuclear cells, 5-HETE and 5-oxoETE were secreted during intermittent hypoxia depending on hypoxia-inducible factor-1. When mice were exposed to intermittent hypoxia, 5-HETE and 5-oxoETE were excreted more in urine. Conclusions: 5-HETE and 5-oxoETE were identified and verified as new OSA markers reflecting hypoxic stress. The OSA markers could be used for OSA screening. |
| Databáze: | OpenAIRE |
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