Cytoprotective Effect of Ulinastatin on LLC-PK1 Cells Treated with Antimycin A, Gentamicin, and Cisplatin
| Autor: | Tomoaki Shinkawa, Masahiro Mizota, Masanori Nakakuki, Fumiaki Yamasaki, Masaaki Ishibashi |
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| Rok vydání: | 1997 |
| Předmět: |
Cisplatin
biology urogenital system business.industry Aminoglycoside General Medicine Antimycin A Pharmacology Ulinastatin Nephrotoxicity chemistry.chemical_compound chemistry Biochemistry Nephrology Lactate dehydrogenase medicine biology.protein Bovine serum albumin business Antibacterial agent medicine.drug |
| Zdroj: | Nephron. 76:300-306 |
| ISSN: | 1423-0186 0028-2766 |
| Popis: | The cytoprotective effect of ulinastatin was studied in LLC-PK1 cells treated with antimycin A, gentamicin, or cisplatin. All of the three agents induced a concentration-dependent increase in the release of lactate dehydrogenase and a decrease in the amount of remaining protein. In the cell injury models treated with 1.5 microM antimycin A, 10 mM gentamicin, and 0.3 mM cisplatin, ulinastatin tended to show a cytoprotective effect at a concentration of 3,000 U/ml and provided a significant protective effect at 10,000 U/ml. LLC-PK1 cells treated with 0.3 mM cisplatin, bovine serum albumin, and alpha1-acid glycoprotein at a concentration of 3.54 mg/ml, which is a comparable protein concentration to that of 10,000 U/ml ulinastatin, showed no protective effect but rather enhanced cell injury. These results suggest that ulinastatin exerts a direct protective effect on LLC-PK1 cells against various renal toxicities. |
| Databáze: | OpenAIRE |
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