Development of a genetic sexing strain ofAnopheles arabiensisfor KwaZulu-Natal, South Africa
| Autor: | Leonard C. Dandalo, Maria L Kaiser, Lizette L. Koekemoer, Givemore Munhenga |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Veterinary medicine General Veterinary Strain (biology) 030231 tropical medicine Anopheles Sexing Biology Y chromosome biology.organism_classification 03 medical and health sciences Mosquito control Dieldrin chemistry.chemical_compound Sterile insect technique 030104 developmental biology 0302 clinical medicine chemistry Insect Science Genotype Parasitology Ecology Evolution Behavior and Systematics |
| Zdroj: | Medical and Veterinary Entomology. 32:61-69 |
| ISSN: | 0269-283X |
| DOI: | 10.1111/mve.12264 |
| Popis: | An efficient sexing system is important for the release of sterile males for any control programme using the sterile insect technique. This study describes the development and characterization of a new genetic sexing strain from South Africa (GMK), needed for the planned implementation of such a programme in northern KwaZulu-Natal Province. The base colony used was a locally modified laboratory strain of Anopheles arabiensis containing a sex-linked gene conferring dieldrin resistance to male mosquitoes. Female A. arabiensis mosquitoes from northern KwaZulu-Natal were mated with these males and backcrossed to introduce the dieldrin resistance gene to the Y chromosome. The resulting strain therefore had an overall genotype representing the local population but with the Y chromosome containing the dieldrin resistance gene. Life-history characteristics, stability of the sex-linked resistance marker, and reduction in dieldrin waste were investigated. The strain showed semi-sterility exhibited by low egg hatch rates, faster development in the immature stages and longer adult survivorship compared with the parental strains. While the GMK strain carrying the dieldrin-resistant gene was successfully established, the stability of the gene is limited, requiring periodic purification. Dieldrin waste can be limited by treating many more eggs than currently recommended. |
| Databáze: | OpenAIRE |
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