High performance liquid chromatography of non-protein amino acids extracted fromAcacia seeds
| Autor: | Christine S. Evans, David A. Younie, Ajit J. Shah, Max W. Adlard |
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| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Acacia Plant Science General Medicine Chloroformate biology.organism_classification Biochemistry High-performance liquid chromatography Analytical Chemistry Amino acid chemistry.chemical_compound Ethanol extracts Complementary and alternative medicine chemistry Drug Discovery Molecular Medicine Gradient elution Organic chemistry Acetonitrile Food Science Precolumn derivatization |
| Zdroj: | Phytochemical Analysis. 3:20-25 |
| ISSN: | 1099-1565 0958-0344 |
| DOI: | 10.1002/pca.2800030104 |
| Popis: | A sensitive, reversed phase, high performance liquid chromatographic procedure for the analysis of non-protein amino acids has been developed. The method is based on precolumn derivatization with 9-fluoroenylmethyl chloroformate of the non-protein amino acids characteristic of seeds of Acacia species, namely N-acetyldjenkolic acid, diaminopropionic acid and diaminobutyric acid and their acetyl, ureido and oxalyl N-substituted derivatives. The separation of derivatives was achieved by means of a gradient elution programme utilizing two buffers of different pH values and acetonitrile. Following separation, the derivatized amino acids were detected spectrofluorometrically. The levels of non-protein amino acids in seeds of Acacia species, which were representative of three taxonomically distinct groups, were measured by analysis of ethanol extracts. |
| Databáze: | OpenAIRE |
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