| Autor: |
Wenhao Yu, Xinlu Zhao, Abubakar S. Jalloh, Yingying Zhao, Brandon Dinner, Yang Yang, Shian Ouyang, Yachao Li, Tian Tian, Zihan Zhao, Rong Yang, Mingkuan Chen, Gregoire Lauvau, Zijian Guo, Peng Wu, Jie P. Li |
| Rok vydání: |
2022 |
| Popis: |
Despite the rich information of a cell’s physiological state encoded in the dynamic changes of cell-surface glycans, methods of capturing glycosylation states at the single-cell level are quite limited. Here we report a chemoenzymatic single-cell N-acetyllactosamine (LacNAc) detection method via tagging the LacNAc with a specific DNA barcode. Compared to the lectin-based glycan detection, the chemoenzymatic labeling does not change the transcriptional status of immune cells and is more compatible with scRNA-seq. Integrated analysis of LacNAc and transcriptome of T cells at a single-cell level reveals that the quantity of cell-surface LacNAc is significantly upregulated in activated CD8+ T cells but maintained at the basal level in quiescent CD8+ T cells (i.e., naive and central memory T cells). Further analysis confirms that the LacNAc level is positively correlated to the glycolytic activity of CD8+ T cells at all statues. Taken together, our study demonstrates the feasibility of chemoenzymatic detection of cell-surface glycan in single-cell RNA sequencing-based multiomics with information of TCR sequence and cell-surface epitopes (i.e., scTCR and CITE-seq) and offers a new way to characterize the biological role of glycan in diversified physiological states. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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