| Autor: |
Gisèle Préaux, Constant Gielens, Lj Verschueren, René Lontie |
| Rok vydání: |
1981 |
| Předmět: |
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| Zdroj: |
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 69:455-462 |
| ISSN: |
0305-0491 |
| DOI: |
10.1016/0305-0491(81)90335-7 |
| Popis: |
1. 1. Chromatography on agarose gels at pH 5.5 in the presence of 1 M NaCI allowed to separate the total β-haemocyanin(β-Hc) of Helix pomatia from the α-haemocyanin (α-Hc). 2. 2. When crystalline β-Hc (β c -Hc) was first insolubilized by dialysis at pH 5.3 at low ionic strength the chromatographic separation yielded the soluble β-haemocyanin fraction (β 3 -Hc) and α-Hc. 3. 3. The amount of β c -Hc varied from one third to one half of β-Hc with the lowest value for non-hibernating snails. 4. 4. β 2 -Hc, in contrast to β c -Hc, did not differ from α-Hc in its electrophoretic and immunological properties. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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