Developmentally regulated conversion of surface-exposed chitin to chitosan in cell walls of plant pathogenic fungi
| Autor: | Nour Eddine El Gueddari, Una Rauchhaus, Bruno M. Moerschbacher, Holger B. Deising |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Hypha biology Physiology fungi food and beverages macromolecular substances Plant Science Fungus biology.organism_classification Polysaccharide Wheat germ agglutinin Microbiology Cell wall Chitosan chemistry.chemical_compound chemistry Chitin Chitinase biology.protein |
| Zdroj: | New Phytologist. 156:103-112 |
| ISSN: | 1469-8137 0028-646X |
| Popis: | Summary • Conversion of surface-exposed chitin to chitosan in cell walls of in vitro - and in vivo -differentiated infection structures of two rust fungi, the wheat stem rust fungus Puccinia graminis f. sp. tritici and the broad bean rust fungus Uromyces fabae , and of the causal agent of maize anthracnose, Colletotrichum graminicola , were studied. • Epi-fluorescence microscopy with the fluorescence-labeled lectin wheat germ agglutinin (WGA) revealed that surfaces of infection structures formed on the plant cuticle expose chitin, whereas surfaces of structures formed after invading the host do not. • To identify chitin modification by de- N -acetylation, we raised polyclonal antibodies specifically recognizing de- N -acetylated chitosan. These antibodies labeled only those infection structures that differentiate inside the plant, indicating that chitosan is exposed on cell wall surfaces post penetration. • Surface modification of the fungal cell walls by chitin de- N -acetylation is discussed as a fungal strategy to protect cell walls of pathogenic hyphae from enzymatic hydrolysis by host chitinases, and to avoid generation of an auto-catalytic defense response system in the invaded host tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text