Lipidogramme détaillé: évaluation enzymatique du cholesterol, des phospholipides et des glycerides après électrophorèse du plasma sur acétate de cellulose
| Autor: | J.F. Menez, B. Person, Bardou Lg, Leglise D, T. Maudelonde |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
education.field_of_study Chromatography biology Cholesterol Glyceride Biochemistry (medical) Clinical Biochemistry Population Fraction (chemistry) General Medicine Biochemistry chemistry.chemical_compound Enzyme chemistry Concanavalin A Mole biology.protein Oil Red O lipids (amino acids peptides and proteins) education |
| Zdroj: | Clinica Chimica Acta. 118:265-277 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(82)90013-4 |
| Popis: | The specific determination of cholesterol and its evaluation in lipoproteins separated by cellulose acetate electrophoresis is described. Using this technique, the authors extended the specific lipid determinations to phospholipids and glycerides. The precision, linearity, accuracy of the methods are satisfactory for fractions greater than 10%. Phospholipids and cholesterol values in alpha lipoproteins are compared with those obtained in the supernatant by concanavalin A precipitation; the differences between the two methods are not significant. We suggest using these three enzymatic determinations to produce a detailed lipidograph. For a selected population, the lipid values in each fraction (expressed in g/l and in mmol/l) were close to those given in the literature. We compared our lipidograph, expressed in SI units, with the Oil Red O stained lipidograph; the difference was not significant. |
| Databáze: | OpenAIRE |
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