Continuous lipase-catalyzed synthesis of hexyl laurate in a packed-bed reactor: optimization of the reaction conditions in a solvent-free system

Autor: Yue-Horng Yen, Hen-Yi Ju, Cheng-Kang Yang, Chwen-Jen Shieh
Rok vydání: 2009
Předmět:
Zdroj: Journal of Chemical Technology & Biotechnology. 84:29-33
ISSN: 1097-4660
0268-2575
Popis: BACKGROUND: Hexyl laurate has been applied widely in cosmetic industries and is synthesized by chemical methods with problems of cost, environmental pollution, and by-products. In this study, Lipozyme® IM77 (from Rhizomucor miehei) was used to catalyze the direct-esterification of hexanol and lauric acid in a solvent-free system by utilizing a continuous packed-bed reactor, wherein the aforementioned difficulties could be overcome. Response surface methodology (RSM) and three-level-three-factor Box-Behnken design were employed to evaluate the effects of synthesis parameters, such as reaction temperature (45–65 °C), mixture flow rate (0.25–0.75 mL min−1) and concentration of lauric acid (100–300 mmol L−1) on the production rate (µmol min−1) of hexyl laurate by direct esterification. RESULTS: The production rate was affected significantly by the mixture flow rate and lauric acid concentration. On the basis of ridge-max analysis, the optimum synthesis conditions for hexyl laurate were as follows: 81.58 ± 1.76 µmol min−1 at 55 °C, 0.5 mL min−1 flow rate and 0.3 mol L−1 lauric acid. CONCLUSION: The lipase-catalyzed synthesis of hexyl laurate by Lipozyme® IM-77 in a continuous packed-bed bioreactor and solvent-free system was successfully developed; optimization of the reaction parameters was obtained by Box–Behnken design and RSM. Copyright © 2008 Society of Chemical Industry
Databáze: OpenAIRE
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