Molecular taxonomy and phylogeny of entomopathogenic nematode species (Rhabditida: Steinernematidae) by RFLP analysis of the ITS region of the ribosomal DNA repeat unit

Autor:	A.P. Reid, William M. Hominick, Bernard R. Briscoe
Rok vydání:	1997
Předmět:	Genetics Restriction enzyme biology Animal ecology Phylogenetics Agarose gel electrophoresis Parasitology Restriction fragment length polymorphism biology.organism_classification Ribosomal DNA Rhabditida Repeat unit
Zdroj:	Systematic Parasitology. 37:187-193
ISSN:	1573-5192 0165-5752
DOI:	10.1023/a:1005843320923
Popis:	The ITS region of 19 isolates of the genus Steinernema Travassos, 1927 belonging to 17 species was PCR amplified. The resulting products were then digested with 17 different restriction endonucleases and the fragments generated were separated by agarose gel electrophoresis. Some enzymes yielded patterns which were highly diagnostic (e.g. Alu I, Dde I, Hha I and Hinf I) while others showed similar RFLP patterns for the majority of species (e.g. Hpa II). All of the species could be unequivocally identified using this method. A tree was constructed based on band sharing which resulted in clusters of species which exhibited similar morphological features.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::22f67dc4bbb6bef840d29134407e31fd https://doi.org/10.1023/a:1005843320923 Zobrazit plný text záznamu Full text from SpringerLink