Paeonol promotes arylamine N‐acetyltransferase activity in human bladder tumor cells
| Autor: | Chin C. Yeh, Huei L. Chang, Hsueh F. Lu, Jing-Gung Chung, Chi F. Hung, Lii T. Wu, Feng T. Yeh |
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| Rok vydání: | 1999 |
| Předmět: |
endocrine system
Chemistry Health Toxicology and Mutagenesis fungi Human bladder N-acetyltransferase Tumor cells NAT activity Pollution Molecular biology High-performance liquid chromatography body regions chemistry.chemical_compound Biochemistry Nat Environmental Chemistry Paeonol Arylamine N-acetyltransferase activity |
| Zdroj: | Toxicological & Environmental Chemistry. 71:211-219 |
| ISSN: | 1029-0486 0277-2248 |
| DOI: | 10.1080/02772249909358793 |
| Popis: | Paeonol was used to determine effects of arylamine N‐acetyltransferase (NAT) activity in human bladder tumor cells. The NAT activity was measured by high performance liquid chromatography assaying for the amounts of N‐acetyl‐2‐aminofluorene (2‐AAF) and N‐acetyl‐p‐amino‐benzoic acid (N‐Ac‐PABA) and remaining 2‐aminofluorene (2‐AF) and p‐aminobenzoic acid (PABA). The NAT activity in human bladder tumor cells was promoted by paeonol in a dose‐dependent manner, i.e. the higher the concentration of paeonol, the higher the promotion of NAT activity. The data also indicated that paeonol increases the apparent values of Km and Kmax from human bladder tumor cells in cytosols and intact cells. This report is the first demonstration to show paeonol did promote human bladder tumor cell NAT activity. |
| Databáze: | OpenAIRE |
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