A mitochondria-targeting ratiometric fluorescent probe for imaging hydrogen peroxide with long-wavelength emission and large Stokes shift
| Autor: | Wenqiang Chen, Xingjiang Liu, Yun Zhang, Xiangzhi Song, Lei Yang, Yani Geng, Long He, Qian Fang |
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| Rok vydání: | 2018 |
| Předmět: |
02 engineering and technology
Mitochondrion 010402 general chemistry Photochemistry 01 natural sciences chemistry.chemical_compound symbols.namesake Stokes shift Materials Chemistry Electrical and Electronic Engineering Hydrogen peroxide Instrumentation chemistry.chemical_classification Reactive oxygen species Metals and Alloys Colocalization 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Benzothiazole Intramolecular force symbols 0210 nano-technology |
| Zdroj: | Sensors and Actuators B: Chemical. 276:247-253 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2018.08.119 |
| Popis: | Hydrogen peroxide, one of the curial reactive oxygen species, is believed to involve in a wide range of physiological processes. In this work, we designed and synthesized a new fluorescent probe, HKB, for the detection of hydrogen peroxide, by taking advantage of the combination of ICT and ESIPT mechanisms. Different from other benzothiazole-based ratiometric probes, the intramolecular ESIPT process is preserved in probe HKB and the large Stokes shift is remained. Probe HKB displayed significant ratiometric fluorescence variations (I594nm/ I666nm) upon the addition of 50 equiv. of hydrogen peroxide under a single-wavelength excitation at 440 nm in PBS buffer (pH = 7.0, 10 mM, 30% CH3CN) and showed a large Stokes shift change from 102 nm to 254 nm. Moreover, this probe exhibited excellent mitochondria targeting property with a 0.94 Pearson’s colocalization coefficient using Rh123 as a reference. Gratifyingly, preliminary biological experiments indicated that probe HKB could sense both the exogenous and endogenous hydrogen peroxide in living HeLa cells. |
| Databáze: | OpenAIRE |
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