Quantitative determination of duloxetine and its metabolite in rat plasma by HPLC-MS/MS
| Autor: | Ho-il Kang, Jung-woo Chae, Hyun-moon Baek, Kwang-il Kwon, Sang Kyum Kim |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Chromatography Chemistry Elution Electrospray ionization Metabolite Clinical Biochemistry General Medicine Mass spectrometry Tandem mass spectrometry Biochemistry Analytical Chemistry chemistry.chemical_compound Liquid chromatography–mass spectrometry Drug Discovery Protein precipitation Glucuronide Molecular Biology |
| Zdroj: | Biomedical Chromatography. |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.2895 |
| Popis: | The major metabolite of duloxetine is a glucuronide conjugate of 4-hydroxy duloxetine (4-HD). However, interestingly, there have been no reports determining concentrations of 4-HD and no fully validated method has been established for measuring duloxetine and 4-HD in rat plasma. We developed a method for the simultaneous quantification of duloxetine and its metabolite in rat plasma using high-performance liquid chromatography tandem mass spectrometry. Duloxetine and 4-HD were analyzed on a reverse-phase C18 analytical column after protein precipitation of the plasma sample with methanol, using carbamazepine as an internal standard. The isocratic mobile phase of 5 mm ammonium acetate-methanol (4:6, v/v) was eluted at 0.4 mL/min. Quantification was performed on a triple-quadrupole mass spectrometer using electrospray ionization, and the ion transition monitored in selective reaction monitoring mode. The coefficient of variation for assay precision was |
| Databáze: | OpenAIRE |
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