Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease1

Autor:	Yota Takahashi, Teruyuki Kodama, Naoya Takeda, Makoto Komiyama, Kazunari Matsumura
Rok vydání:	1994
Předmět:	Nuclease biology Stereochemistry chemistry.chemical_element General Chemistry Ligand (biochemistry) chemistry.chemical_compound Cerium chemistry Ribose Phosphodiester bond biology.protein Organic chemistry Thymidine DNA Bond cleavage
Zdroj:	Supramolecular Chemistry. 4:31-34
ISSN:	1029-0478 1061-0278
DOI:	10.1080/10610279408029859
Popis:	Cerium(IV) ion is attached to the 5′-end of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidylyl(3′-5′)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.
Databáze:	OpenAIRE
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