| Autor: | Lauren M. McIntyre, Richard P. Westerman, Damion Junk, Lisa M. Bono, Artyom Kopp, Marina Telonis-Scott, Sergey V. Nuzhdin, Marta L. Wayne, Anne Genissel, Larry Harshman |
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| Rok vydání: | 2006 |
| Předmět: |
Genetics
0303 health sciences biology Alternative splicing Genomics biology.organism_classification Transcriptome Gene expression profiling 03 medical and health sciences 0302 clinical medicine Gene expression Drosophila melanogaster Gene 030217 neurology & neurosurgery X chromosome 030304 developmental biology |
| Zdroj: | Genome Biology. 7:R79 |
| ISSN: | 1465-6906 |
| DOI: | 10.1186/gb-2006-7-8-r79 |
| Popis: | Background: Many genes produce multiple transcripts due to alternative splicing or utilization of alternative transcription initiation/termination sites. This 'transcriptome expansion' is thought to increase phenotypic complexity by allowing a single locus to produce several functionally distinct proteins. However, sex, genetic and developmental variation in the representation of alternative transcripts has never been examined systematically. Here, we describe a genome-wide analysis of sex-specific expression of alternative transcripts in Drosophila melanogaster. Results: We compared transcript profiles in males and females from eight Drosophila lines (OregonR and 2b, and 6 RIL) using a newly designed 60-mer oligonucleotide microarray that allows us to distinguish a large proportion of alternative transcripts. The new microarray incorporates 7,207 oligonucleotides, satisfying stringent binding and specificity criteria that target both the common and the unique regions of 2,768 multi-transcript genes, as well as 12,912 oligonucleotides that target genes with a single known transcript. We estimate that up to 22% of genes that produce multiple transcripts show a sex-specific bias in the representation of alternative transcripts. Sexual dimorphism in overall transcript abundance was evident for 53% of genes. The X chromosome contains a significantly higher proportion of genes with female-biased transcription than the autosomes. However, genes on the X chromosome are no more likely to have a sexual bias in alternative transcript representation than autosomal genes. Conclusion: Widespread sex-specific expression of alternative transcripts in Drosophila suggests that a new level of sexual dimorphism at the molecular level exists. |
| Databáze: | OpenAIRE |
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