| Autor: |
Stephen C. Whisson, Kathryn M. Wright, A. Thirugnanasambandam, Adrian C. Newton, N. D. Havis |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Plant Pathology. 60:929-937 |
| ISSN: |
0032-0862 |
| Popis: |
Ramularia collo-cygni, the causal agent of ramularia leaf spot disease in barley was transformed to express the green fluorescent protein and DsRed fluorescent protein using a combined protoplast and Agrobacterium tumefaciens-mediated transformation technique. Regenerated protoplasts from mycelial fragments were transformed with A. tumefaciens strain AGL1 containing either pCAMBgfp or pCAMBDsRed plasmids. Two different isolates of R. collo-cygni were transformed and several stable transformants were recovered. These transformants infected detached barley leaves and characteristic swan’s-neck-shaped conidiophores were seen on detached leaves 9 days after inoculation, as well as spore-like structures formed from hyphal swellings. This first successful transformation of R. collo-cygni has enabled better description of the stages of disease development and pathogen behaviour during the endophytic/asymptomatic stage of its life cycle. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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